Protein Expression, Purification and Characterization
NAB offers different methods of protein purification for our customers to determine the type and level of purity wanted for their applications. We use both bacterial and mammalian cell expression systems to produce recombinant proteins in an economical way, with native confirmation (complete post-translational modifications and proper foldings). NAB’s expert knowledge in molecular biology and protein biochemistry will provide cost effective recombinant protein synthesis to their valued customers. We design and construct the vector to produce optimal protein quantity and quality. The problems associated with protein expression will be eliminated by all the available genetic engineering tools. We have extensive experience in purifying a wide variety of recombinant proteins either with/without tag (for eg. His, GST). Our protein purification techniques include Affinity, Gel Filtration, Ion Exchange and Size Exclusion Chromatography. We characterize the purified protein by poly acrylamide electrophoresis, staining and Western blotting. If our customers need further characterization, formulation and stabilization, we will arrange for it. We will deliver custom made proteins, analysis data, QC reports and DNA constructs. NAB can procure commercially available expression vectors of customer’s choice, clone in customer’s gene of interest, express and purify according to the needs of our customers.
Our standard services include
- Initial discussion about selecting expression vector, system, etc.
- Cloning in expression vector
- Transformation or transfection into bacterial or mammalian cells
- Protein expression and optimization
- Protein purification and characterization,
- Delivering purified protein, expression vector map and quality control data
We can also provide the following for an additional fee
- Frozen stock of transformed cells
- Protein expressed cell pellet
- Conditioned media
To place an order, please contact us or fax a quote request form to 1-614-573-7457.
Protein Research Reagents